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Toxicity of tire debris extracts on human lung cell line A549

TitoloToxicity of tire debris extracts on human lung cell line A549
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2005
AutoriGualtieri, Maurizio, Rigamonti L., Galeotti V., and Camatini M.
RivistaToxicology in Vitro
Volume19
Paginazione1001-1008
ISSN08872333
Parole chiaveCell cycle, Cell Line, cell structure, Cell Survival, Comet Assay, conference paper, controlled study, Cytotoxicity, DNA damage, Dose-Response Relationship, Drug, Environmental Pollutants, evaluation, human, human cell, Humans, lung alveolus cell, motor vehicle tire, particle size, Pulmonary Alveoli, respiratory system, Rubber, Time Factors
Abstract

TD, produced by tire wear, is a significant constituent of PM10 in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 μg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 μg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose. © 2005 Elsevier Ltd. All rights reserved.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-27644532408&doi=10.1016%2fj.tiv.2005.06.038&partnerID=40&md5=aa8e7579701b02c8ea2077181b8badd4
DOI10.1016/j.tiv.2005.06.038
Citation KeyGualtieri20051001