| Title | Toxicity of tire debris extracts on human lung cell line A549 |
|---|---|
| Publication Type | Articolo su Rivista peer-reviewed |
| Year of Publication | 2005 |
| Authors | Gualtieri, Maurizio, Rigamonti L., Galeotti V., and Camatini M. |
| Journal | Toxicology in Vitro |
| Volume | 19 |
| Pagination | 1001-1008 |
| ISSN | 08872333 |
| Keywords | Cell cycle, Cell Line, cell structure, Cell Survival, Comet Assay, conference paper, controlled study, Cytotoxicity, DNA damage, Dose-Response Relationship, Drug, Environmental Pollutants, evaluation, human, human cell, Humans, lung alveolus cell, motor vehicle tire, particle size, Pulmonary Alveoli, respiratory system, Rubber, Time Factors |
| Abstract | TD, produced by tire wear, is a significant constituent of PM10 in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 μg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 μg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose. © 2005 Elsevier Ltd. All rights reserved. |
| Notes | cited By 33 |
| URL | https://www.scopus.com/inward/record.uri?eid=2-s2.0-27644532408&doi=10.1016%2fj.tiv.2005.06.038&partnerID=40&md5=aa8e7579701b02c8ea2077181b8badd4 |
| DOI | 10.1016/j.tiv.2005.06.038 |
| Citation Key | Gualtieri20051001 |
